本研究根据转录组信息从甘蔗细茎野生种(Saccharum Spontaneum)中克隆得到一个抗旱相关的NAC家族基因，命名为ScNA C1(NCBI accession KP742345)。研究结果显示：该基因包含一个1212个碱基的开放阅读框，编码403个氨基酸；通过序列比对发现该基因在N端具有保守的NAC结构域，进化树分析显示Sc NA C1属于NAC家族基因的NAP亚家族；通过将ScNAC1-GFP融合载体转化拟南芥原生质体中，证明Sc NA C1定位于细胞核内；定量PCR检测在甘蔗细茎野生种发育不同时期Sc NA C1对于干旱的响应，发现Sc NA C1在各个时期都受干旱诱导。研究结果可为进一步研究甘蔗干旱响应机理提供参考。
Sac c harum Sp ontane um Sc NA C1 Clone and Funtion Analysis
In this study, a new drought related NAC family gene was cloned from India Saccharum Spontaneum named ScNA C1 (NCBI accession KP742345). This gene contained 1 212 bp open reading frame, coding 403 amino acids; Homologous gene alignment results showed conserved NAC domain in N terminal of ScNA C1, and phylogenetic tree analysis showed it belonged to NAP subfamily of NAC family. Arabidopsis protoplast transformation of ScNAC1-GFP fused vector demonstrated ScNA C1 subcellular located in nuclear. Real time quantitative PCR results shown ScNAC1 can be induced by drought in any developmental stage of Saccharum Spontaneum. This study laid foundation for deep understanding of drought-response mechanism of sugarcane.
India Saccharum SpontaneumDroughtScNAC1Function analysis
NETL
NSTL
万方数据
