首页|黄瓜扩展蛋白基因家族的鉴定与生物信息学分析

黄瓜扩展蛋白基因家族的鉴定与生物信息学分析

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扩展蛋白(expansins)是植物细胞壁的重要组成部分,能够引起细胞壁组分间松驰和细胞壁柔韧性增加,在植物的许多生长发育过程中发挥作用.为了揭示黄瓜扩展蛋白基因家族的功能,本研究利用全基因组测序数据鉴定黄瓜扩展蛋白基因家族,分析其结构特征及系统发育关系.黄瓜基因组测序数据分析显示,黄瓜扩展蛋白基因家族包含35个成员,包括A (21)、B(3)、LA (9)和LB (2)4个亚家族,分布在黄瓜7条染色体上.扩展蛋白氨基酸长度范围为206-295,编码蛋白质具有保守的结构域,蛋白质亚细胞定位预测结果表明所有蛋白均定位于细胞外.基因结构分析表明,黄瓜扩展蛋白四个亚家族的平均内含子数目分别为A类1.8个,B类2.7个,LA类3.8个和LB类3.5个.基因表达分析发现,该家族基因在黄瓜雌雄花蕾及果实发育过程中表达丰度较高.本研究分析了黄瓜扩展蛋白基因家族的基本信息,可为深入研究其扩展蛋白基因的分子进化与生物学功能奠定基础.
Identification and Bioinformatic Analysis of the Expansin Gene Family in Cucumber
As an important component of plant cell wall, expansins have effects on loosening and increasing the flexibility of cell wall, and are thought to have function in many process of plant growth and development.To study potential function ofexpansin gene family in cucumber, we investigated expansin gene family with the whole genome database to analyse its structure and phylogenetic relationship.Whole genome sequencing of cucumber revealed it contained 35 expansin genes which belonged to 4 subfamilies (EXPA (21), EXPB (3), EXLA (9) and EXLB (2)) located on 7 different chromosomes.Each of expansin proteins ranged from 206 aa to 295 aa in size and had conserved domains.All 35 expansin proteins were predicted to localize in extracellular.Gene structure analysis revealed that average intron number of four subfamily was 1.8 (EXPA), 2.7 (EXPB), 3.8 (EXPA) and 3.5 (EXPB),respectively.Transcriptome analysis showed that the expansin genes expressed highly in female and male flower buds, and fruit development process.The results here displayed the basic information of cucumber expansin superfamily which could be a good platform for the gene evolution and function study in future.

Cucumis sativus L.ExpansinBioinformaticsGene family

郝西、理向阳、腊贵晓、代丹丹、杨铁钢

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河南省农业科学院经济作物研究所,农业部中原地区作物栽培科学观测实验站,郑州,450002

黄瓜 扩展蛋白 生物信息学 基因家族

河南省农业科学院自主创新项目国家星火计划项目河南省农业科学院优秀青年科技基金

2013ZZ172013GA7500012013YQ09

2015

分子植物育种
海南省生物工程协会

分子植物育种

CSTPCDCSCD北大核心
影响因子:0.765
ISSN:1672-416X
年,卷(期):2015.13(10)
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