Establishment of Embryogenic Cell Suspension Culture and Plant Regeneration of Rare Endangered Plant Tapiscia sinensis Oliv
We preliminary established embryonic cells suspension system and plant regeneration system by exploring the best conditions of embryogenic callus abduction and embryogenic cells cultivation,with Tapiscia sinensis Oliv immature seed tree as an experimental material.Embryogenic callus could be originated from immature seeds on MS solid medium augmented with 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 g/Lactive carbon.Obtained callus were subsequently cultured in MS liquid medium with 0.2 mg/L 6-benzylaminopurine (6-BA),0.05 mg/L naphthalene acetic acid (NAA) and 3 g/L sucrose for establishing rapid-growing and well-dispersed embryogenic cell suspension system.The cotyledon embryos from suspension culture could grow into normal plants in liquid MS medium without any plant growth regulating substances.
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维普
