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滇西北玉龙雪山不同海拔川滇高山栎遗传多样性分析

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本研究滇西北玉龙雪山川滇高山栎自然居群的遗传多样性,以保护和合理利用川滇高山栎资源.利用SSR分子标记对滇西北玉龙雪山不同海拔(2750~3500 m)川滇高山栎(Quercus aquifolioides)6个居群进行遗传多样性分析.结果表明川滇高山栎在物种水平上Shannon多样性指数(I)为1.64,Nei's基因多样性指数(He)为0.73,反映出川滇高山栎总的遗传多样性丰富,处于较高水平.由Shannon信息指数计算的值比Nei指数估算的居群遗传多样性高,但两者都一致表明6个居群的遗传多样性水平随海拔梯度的变化而表现相同的变化规律:海拔2750m至3200 m川滇高山栎的遗传多样性随海拔的增高而增高,而海拔3200 m至3500m居群的遗传多样性水平有降低的趋势.川滇高山栎的Fst为0.09,表明9.00%遗传变异存在于居群间,而91.00%的遗传变异存在于居群内,AMOVA分析表明13.63%的变异存在于居群间,86.37%的变异存在于居群内,两者都说明川滇高山栎居群间存在较低的遗传分化,总的遗传分化主要来自居群内部.基因流(Nm)为2.44,表明较高的基因流可能是导致川滇高山栎具有较高的遗传多样性的主要因素之一.研究结果为川滇高山栎资源保护等研究提供了理论依据.
Analysis on the Genetic Diversity of Quercus aquifolioides at Different Altitudes
Our objective was to evaluate the genetic diversity of six populations of Quercus aquifolioides which distributed in different altitudes (2750~3500 m) of Jade Dragon Snow Mountain in the northwest of Yunnan Province.6 populations including 132 individuals at different altitudes were analyzed by simple sequence repeat (SSR) markers.The results showed that Shannon index (Ⅰ) and the Nei's gene diversity index (He) was 1.64 and 0.73,respectively,indicating Quercus aquifolioides had high genetic diversity at species level.The genetic diversity calculated by I was higher than estimated by He,but both of them agreed that the genetic diversity of six population varied with different altitude gradients:the level of genetic diversity from the altitudes of 2750 m to 3200 m was high,and it decreased at the altitude of 3200 m to 3500 m.Nei's genetic differentiation of Quercus aquifolioides Fst was 0.09 and AMOVA analysis (Phist=0.1363),indicating that a low level of genetic differentiation among populations.Gene flow among populations was 2.44,suggesting that high gene flow may be a main factor leading to high levels of the genetic diversity among populations.

Genetic differenceGenetic diversitySSRQuercus aquifolioides

程小毛、王振章、姜永雷、黄晓霞

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西南林业大学园林学院,昆明,650224

河南省安阳县农业局,安阳,455000

中国科学院成都山地灾害与环境研究所山地表生过程与生态调控重点实验室,成都,610041

遗传分化 遗传多样性 SSR 川滇高山栎

国家自然科学基金云南省自然科学基金云南省省级重点学科园林植物与观赏园艺建设经费

311002922010ZC26750097401

2016

分子植物育种
海南省生物工程协会

分子植物育种

CSTPCDCSCD北大核心
影响因子:0.765
ISSN:1672-416X
年,卷(期):2016.14(5)
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