Floral Transcriptome Sequencing and Characteristic Analysis of Cymbidium tortisepalum
Cymbidium tortisepalum belongs to a kind of precious and pretty orchid in China.However there is little known about its molecular biology.Both Illumina HiSeqTM 2000 sequencing platform and transcriptome analysis were employed to reveal the molecular mechanism of Cymbidium tortisepalum.After removing low quality sequences,Clean reads were assembled into 57 011 Unigenes.Among these unigenes,24 049 unigene were annotated based on Nr,Swiss-prot,COG and KEGG public databases respectively.About 32 962 Unigene gene didn't get comment can be considered to be C.tortisepalum new transcripts and specific genes.36 CDS genes encode as MA DS-box gene,its transcription factors involve in flowering development based on our Illumina data set.Phylogenetic tree showed that 23 MADS genes were classified into class A,class B,class C,class D and class E subfamily,which involved in development of floral organ,and 8 MADS genes were related to blooming time.RPKM analysis indicated that PI,AP3 and A GL6 expressed much higher in flower than other MADS genes.This study provides a theoretical basis on the mechanism of flower development and blooming of Cymbidium tortisepalum.
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