为探索百合的耐盐,耐寒和耐旱的抗性机理,本试验选取了抗寒能力极强的岷江百合(Lilium regale)为材料,利用RT-PCR技术克隆得到了岷江百合的LiGPAT基因的ORF区序列,全长1 212bp,编码403个氨基酸,分子量约为45.4 kD,GenBank登录号为JX524740.通过同源序列比对发现,LiGPA T基因编码的氨基酸与单子叶植物油棕GPAT基因编码的氨基酸的一致性最高,达到67.17％.利用在线软件SMART进行分析,表明LiGPAT氨基酸在169～315序列存在一个PlsC结构域,这就说明LiGPAT蛋白属于酰基转移酶家族成员,且所有的活性位点都位于这个区域,具有酰基转移酶的活性.通过在线软件TMHMM Server 2.0预测分析揭示LiGPAT氨基酸序列不存在跨膜区域,可能是一个可溶性蛋白.利用在线软件Plant-mPLoc预测表明,LiGPAT蛋白质的亚细胞定位为叶绿体.
Cloning and Bioinformatics Analysis of Lilium regale LiGPA T Gene
In this research we chose lily (Lilium regale) which had high cold resistance as the material to explore the resistance mechanism of salt tolerance,cold tolerance and drought tolerance.We obtained the ORF sequence ofLiGPA T gene using RT-PCR technology.The full length sequence contains 1 212 bp nucleotids,encoding 403 amino acid,and its molecular weight is 45.4 kD.GenBank accession number was JX524740.Multiple sequence alignment and phylogeny evolution analysis showed that Lilium regale shared 67.17％ sequence homology with Elaeis guineensis.The prediction result of SMART suggested that a domains PlsC existed in LiGPA T amino acid.LiGPAT protein was a member of the family of acyltransferase.And all the active sites with acyltransferase activity were located in this area.Forecast analysis revealed that LiGPA T amino acid did not have transmembrance domain,and suggested that LiGPA T was a water-soluble protein by TMHMM Server 2.0.Prediction of the subcellular localization indicates that LiGPAT protein was distributed in the chloroplast by Plant-mPLoc online software.
NETL
NSTL
万方数据
维普
