Cloning and Bioinformatics Analysis of GPAT9 Gene in Rapeseed
GPAT9 is one of the important genes in fat metabolism of plants,but the research of this gene is not in-depth in Brassica napus.Studying the GPAT9 gene of Brassica napus,we designed a pair of primers,and according to the homologous comparsion of B.campestris,B.oleracea and A rabidopsis thaliana GPAT9 sequence,we cloned a full length CDs sequence that has 1 131 bp and that we named BnGPA T9.BnGPAT9 encodes a protein of 376 amino acids with the molecular weights of 43.25 kD,the instability index of 46.46 and the isoelectisc point of 8.97,and it encodes a protein of three TMHs without SignalP.The second structure of BnGPAT9 has 38.03％ alpha helix,21.28％ beta sheet,9.04％ turn and 31.65％ coil.Phylogenic tree analysis of BnGPAT9 indicated that BnGPA T9 was closely related to the GPAT9 in B.campestris,B.oleracea and Arabidopsis thaliana.BnGPAT9 possesses LPCAT1-like in Lysophospholipid acyltransferase superfamily and GPAT9 motifs.In terms of sequence comparsion,the analysis of physic-chemical property,subcellular localization and conserved domain,they showed that BnGPAT9 was Xiangyou 15 GPA T9 gene,and it should be the members of GPA Ts.
NETL
NSTL
万方数据
维普
