ISSR Analysis on Genetic Diversity of Different Varieties (Lines) in Solanum tuberosum L.
In this research,we used the ISSR molecular marker,in order to make polymorphism,cluster analysis and analyse the genetic relationships of 30 different varieties (lines) in Solanum tuberosum.The results showed that:After amplified 6 primers from 56 ISSR primer pools,total 52 DNA bands were amplified,of which 47 were polymorphic,and the polymorphic rate was 90.38%.Genetic similarity coefficient of 30 potato varieties (strains) changed from 0.40-0.95,with an average of 0.675.Cluster analysis results indicated that the potato varieties (strains) could be classified into 6 groups at 0.75 similarity level,including 5 composite groups and 1 independent group.These results indicated that 30 Solanum tuberosum varieties strains of the selected genetic differences,and the genetic diversity was abundant.In addition,the results of this study provided a strong basis for the utilization of genetic diversity,germplasm identification,and cross-breeding of Solanum tub erosum.