Genetic relationship of 4 Cymbidium varieties was analyzed via PCR amplication and contruction of phylogenetic tree.Results showed:(1) Length of four varieties on ITS site were among 647 bp to 655 bp,the ITS 1 had 242 bp,and ITS2 had 244 bp.(2) 175 bp variation sites were existed in four varieties,and the ITS1 and ITS2 region had high variation.(3) Content of(G+C) were among 60.12％ to 68.21％,C.wenshanense had the highest content,and C.cochleare got the opposite result.These results illustrate the ITS gene can be seen the marker to studyphylogenetic relationship,and has high sensitivity,could be offered exact support for Cymbidium phylogenetic study.
NETL
NSTL
万方数据
维普
