Cloning and Expression Analysis of GGPPS Gene from Mango (Mangifera indica)
Geranylgeranyl diphosphate (GGPP) is the precursor for the biosynthesis of gibberellins,carotenoids,chlorophylls,isoprenoid quinones and geranylgeranylated proteins in plants.A MinGGPPS1 gene was cloned from mature mango fruit by using cDNA as template through transcriptome RNA-Seq.Through sequencing,multiple alignment and phylogenetics analyses,we found the sequence was likely a GGPPS gene and predicted coding region is the largest ORF,with the full-length cDNA as 1 100 bp.Protein sequence comparison indicated that MinGGPPS1 is closely related (92％) to the MinGPS2 of Mangifera indica.We selected 12 GGPPS genes,2 FPPS genes,2 SPPS genes,and 1 GPS gene in Arabidopsis thaliana as well as other related genes.BLAST comparisons of available GGPPS,FPPS,and GPS sequences revealed the presence of two DDx2-4D motives and a conserved CxxxC motif in each GGPPS sequences.It was found that the protein encoded by the gene had close relationship with GGPPS through Mrbayes analysis.Expression of MinGGPPS1 gene in different tissues and organs are as follows:the high expression of green leaves,and the lower expression in stems,similar amount between unripe and ripe peels.The expression in the ripe flesh was almost five times as much as it was in the unripe flesh,which indicates that GGPPS has the function of regulating flesh ripening.
NETL
NSTL
万方数据
维普
