The Correlation Analysis on SS and SE of Copy Number and Saponins Content in Eleutherococcus senticosus
Be based on the DNA sequence ofsqualene synthase gene 2 (SS2) and squalene epoxidase gene 1 (SE1),using southern blotting and qRT-PCR technique to confirm the copy number of SS2 and SE1 gene.In this research we used the actin gene for reference gene,using qRT-PCR technique and spectrophotometry to confirm gene expression and saponins content of SS2 and SE1 gene in different copy number.The results show that SS2 gene the type of copy number is 1 and 2,and SE1 gene is 1,2,3 and six genotypes by their intercombination.The gene expression and saponins content of SS2 and SE1 gene are improved significantly (P＜0.05) with the improving of copy number in Eleutherococcus senticosus.It has significantly positive correlation (P＜0.01) SS2 and SE1 gene every addional one copy can improve 0.57 and 0.42 times saponins content.Research results established laid the foundation for reveal molecular mechanism that form the difference of saponins content in E.senticosus.
NETL
NSTL
万方数据
维普
