水稻雌性半不育突变体M21的细胞学研究和基因的初步定位
Cytological Studies and Preliminary Mapping of M21, a Female Semi-sterility Mutant in Rice
龙思芳 1张大双 2彭强 2姜雪 2雷月 2宫彦龙 2徐海峰 2李佳丽 2邓如玉 2朱速松2
作者信息
- 1. 贵州师范大学,贵阳,550001
- 2. 贵州省水稻研究所,贵阳,550006
- 折叠
摘要
水稻雌配子的发育直接影响水稻的育性.由于雌配子败育的机理复杂,使得雌性不育的分子机理研究一直未取得突破性进展.本研究以EMS诱变获得的半不育突变体M21为研究材料,采用细胞学技术发现M21是由胚囊部分败育造成的.以M21//M21/N22回交群体BC1F1为作图群体全基因组分析,构建全长为2 674.2 cM的遗传图谱,包含了189对SSR标记,平均遗传距离为14.1 cM;复合区间作图法(CIM)进行QTL定位,在第11号染色体检测到一个新的雌配子半不育QTL qPS-a,初定位在标记RM26761和RM26841之间,其贡献率为20.2％.本研究有助于进一步探究水稻雌性半不育的遗传机制.
Abstract
The development of female directly affects the fertility office (Oryza sativa).The molecular and cellular mechanisms regulating female fertility in rice remain poorly understood as for it's a complex network.In this study,we identified a new stable genetic female semi-sterility mutant M21.Cytological studies showed the reduced spikelet fertility primarily caused by reduced embryo sac viability.A genetic linkage map covering 2 674.2 cM was constructed based on genome-wide analysis using a backcross population of M21//M21/N22,the genetic map contained 189 SSR makers with an average interval of 14.1 cM;a loci on chromosome 11 between makers RM26761 and RM26841 conferring the female semi-sterility was detected,named qPS-a,which explained more than 20％ of the phenotypic variation for female semi-sterility.No sterility related QTLs/genes were reported between these two markers.These results would be beneficial to the further study on the genetic mechanisms of female semi-sterility in Rice.
关键词
水稻/雌配子/半不育/基因定位Key words
Oryza sativa/Female gametophyte/Semi-sterility/Mapping of Genes引用本文复制引用
基金项目
贵州省农业科技体制改革与创新服务园区项目([201619)
贵州省农业科学院研究生科研创新基金(0009)
贵州水稻现代农业产业体系技术体系项目(GZCYTX2016-06)
“十二五”农村领域国家科技计划项目(2014AA10A-604-11)
贵州省科技合作计划项目(7074)
贵州师范大学研究生创新基金(23)
出版年
2017
NETL
NSTL
维普
万方数据