Establishment and Optimization of SRAP-PCR Recation System for Hippeastrum spp.
This research optimized five factors of SRAP-PCR recation system for amaryllis (Hippeastrum spp.) by L16(45) orthogonal design,including concentrations of Mg2+,dNTPs,primers,primer concentration,amounts of DNA template and Taq DNA polymerase.The results showed that influence factors of amaryllis SRAP-PCR amplification were sorted by concentration of dNTPs,concentration of primers,concentration of Mg2+,amount of DNA template and amount of Taq DNA polymerase.The optimized amaryllis SRAP-PCR reaction system was:1×PCR Buffer,2.0 mmol/L Mg2+,0.2 mmol/L dNTPs,0.25μmol/L primer,80 ng DNA template and 0.5 U Taq DNA polymerase in 20 μL system.This optimized system was verified by ten amaryllis varieties,which proved that it was highly steady and repeatable,and it could provide an important technical support in genetic diversity researches and genetic map constructions.
Hippeastrum spp.SRAPOptimization of reaction systemOrthogonal design
NETL
NSTL
万方数据
维普
