Cloning and Expression Analysis of Glucosyltransferase Gene TaUGT73D1 in Wheat
To investigate the role of TaUGT73D1 gene in the formation of wheat stamens and pistils during the development of wheat flower,three homologous genes TaUGT73D1-2A,TaUGT73D1-2B and TaUGT73D1-2D were cloned of TaUGT73D1 (UDP-glycosyltransferase 73D1) from wheat three pistils TP and pistillody mutant HTS-1 in this study.By nucleotide sequence analysis,the results showed that TaUGT73D1-2A,TaUGT73D1-2B,TaUGT73D1-2D were 1 907 bp,1 912 bp,1 905 bp in full-length,respectively.The ORF of TaUGT73D1-2A,TaUGT73D1-2B and TaUGT73D1-2D were 1 365 bp,1 467 bp,1461 bp,and coded for deduced protein of 454,488 and 486 amino acids,respectively.By comparing with the wheat genome,three TaUGT73D1 homologous enes (TaUGT73D1-2A,TaUGT73D1-2B,TaUGT73D1-2D) were isolated and found to be located on chromosomes 2AL,2BL,and 2DL,respectively.The Coding region of TaUGT73D1-2A,TaUGT73D1-2B and TaUGT73D1-2D with TuUGT73D1 are respectively 97.68％,96.52％ and 99.05％ similarity.And the similarity of protein sequence was 73.57％,95.08％ and 95.70％,respectively.Phylogenetic analysis showed that the TaUGT73D1 genes was most closely related to monocots UGT genes,indicating their close genetic relationship.However,it was far from the diocts,which indicated their distant relationship.Real-time PCR was used to study the pattern of TaUG T73D1-2A,TaUGT73D1-2B and TaUGT73D1-2D expression in pistil (P),stamen (S) and pistillody stamen (PS).The results showed that TaUGT73D1-2A,TaUGT73D1-2B and TaUGT73D1-2D had a higher expression levels in Stamen,but very rare in Pistillody,Stamen and Pistil.So we speculated that TaUGT73D1 gene may be related to the homologous transformation of stamen into pistil in wheat.This study provided a theoretical basis for the further study of TaUGT73D1 gene function in wheat.
NETL
NSTL
万方数据
维普
