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美洲黑杨锈菌病原真菌的鉴定及致病性研究

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为了明确美洲黑杨锈病的病原菌种类,本研究从江苏南京采集美洲黑杨锈病样品,对病原物形态进行观察,同时进行致病性测定,并对再次致病的病原物进行形态学观察,从江苏南京、江苏泗洪、湖北武汉、湖北石首采集美洲黑杨锈病样品,进行rDNA-ITS序列分析鉴定.结果表明,通过对美洲黑杨锈菌形态特征进行观察,表明长江中下游地区美洲黑杨锈菌的形态与Melampsora larici-populina相似.致病性测定结果表明,所采集锈菌能引起美洲黑杨锈病,与自然发病症状相同,而且发病后的病原物形态特征与自然采集的病原菌相同.根据ITS序列进行聚类,四地的菌株均与M.larici-populina聚在一起,表明长江中下游地区的美洲黑杨锈病病原菌为M.larici-populina.
Identification and Pathogenicity of Fungus on Populus trichocarpa
Based on morphological studies,artificial inoculation tests and sequence cluster analysis of rDNA-ITS region,the species of poplar (Populus trichocarpa) leaf rust sampled from Nanjing,Sihong,Wuhan,Shishou were determined.The characteristic features of the rust pathogen collected from Nanjing were quite similar to that of Melampsora larici-populina.Artificial inoculation tests proved that the sampled pathogen could induce the symptoms of poplar rust,which was the same as that observed in nature.Finally,the rDNA-ITS sequence cluster analysis showed that the rust pathogen from the four places were all located at the same branch as M.larici-populina in the phylogenetic tree.Thus,the poplar leaf rust that occurred in the middle and lower reaches of Yangtze River was determined to be M.larici-populina.

Populus trichocarpaRustPathogenicityITS sequence

万志兵、方乐成、李怡然、陈赢男

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黄山学院生命与环境科学学院,黄山,245041

南京林业大学林学院,南京,210037

美洲黑杨 锈病 致病性 ITS序列

国家林业公益性行业科研重大项目国家自然科学基金江苏省优势学科

20130410231400563PA-PD

2017

分子植物育种
海南省生物工程协会

分子植物育种

CSTPCDCSCD北大核心
影响因子:0.765
ISSN:1672-416X
年,卷(期):2017.15(7)
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