Identification and Pathogenicity of Fungus on Populus trichocarpa
Based on morphological studies,artificial inoculation tests and sequence cluster analysis of rDNA-ITS region,the species of poplar (Populus trichocarpa) leaf rust sampled from Nanjing,Sihong,Wuhan,Shishou were determined.The characteristic features of the rust pathogen collected from Nanjing were quite similar to that of Melampsora larici-populina.Artificial inoculation tests proved that the sampled pathogen could induce the symptoms of poplar rust,which was the same as that observed in nature.Finally,the rDNA-ITS sequence cluster analysis showed that the rust pathogen from the four places were all located at the same branch as M.larici-populina in the phylogenetic tree.Thus,the poplar leaf rust that occurred in the middle and lower reaches of Yangtze River was determined to be M.larici-populina.