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桔梗高含量多糖种质资源的筛选

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本研究以桔梗(Platycodon grandiflorum)为试材,采用热水浸提法,对10种不同产地的桔梗多糖提取工艺进行优化,通过最优条件和同一条件下提取的桔梗多糖,比较分析各个产地桔梗多糖的提取率及得率的差异.结果显示:十个产地桔梗多糖的提取率大小依次为:吉林>内蒙>云南>河北>湖北>安徽>甘肃>河南>四川>山东,即70.64%>61.34%>56.60%>41.34%>40.65%>33.85%>30.63%>28.48%>27.74%>27.19%.吉林桔梗在各产地桔梗中含糖量较高且较易提取,在50℃、料水比1:20的条件下,提取率为70.64%,多糖得率为19.89%,可作为优质资源加以利用.
Screening for Germplasm Resources of Platycodon grandiflorum with High Polysaccharide Content
In this study,we used Platycodon grandiflorum as materials and optimized the polysaccharide-extracting technology of Platycodon grandiflorum collected from 10 different producing areas by hot water extraction method.We compared the polysaccharide extraction rates of Platycodon grandiflorum from different areas under optimal conditions and the same conditions.The results showed that the polysaccharides extraction rate of platycodon grandiflorum from the ten areas were as follows:Jilin>Inner Mongolia>Yunnan>Hebei>Hubei>Anhui>Gansu>Henan>Sichuan>Shandong,and their extraction rates were 70.64%>61.34%>56.60%>41.34%>40.65%>33.85% >30.63% >28.48% >27.74% >27.19%.The polysaccharide content of Platycodon grandiflorum from Jilin was higher than others and it was easier to extract.The extraction rate of polysaccharide was 70.64%,the yield of polysaccharide was 19.89% under a ratio of 1:20 (material to water) at 50℃,which meant that it might be used as a high-quality resource.

Platycodon grandiflorumPolysaccharideHot water extraction methodScreening resources

杨晓杰、张晶、王瑶、孙百良、王世佳

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齐齐哈尔大学生命科学与农林学院,齐齐哈尔,161006

桔梗 多糖 热水浸提法 资源筛选

黑龙江省教育厅资助项目

12541875

2017

分子植物育种
海南省生物工程协会

分子植物育种

CSTPCDCSCD北大核心
影响因子:0.765
ISSN:1672-416X
年,卷(期):2017.15(10)
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