Bioinformatics Analysis of MDH Structure and Function in Cassava(Manihot esculenta)
Cassava (Manihot esculenta Crantz) is one of the most important staple crops.It has rich starch in storage root.Malate dehydrogenase (MDH) is a key enzyme,which plays an important role in starch and sucrose metabolism.In this study,the composition of amino acids,leader peptides,transmembrane segments,hydropho-bicity or hydrophilicity,2-D and 3-D structures and functional domains of MDH protein in cassava were analyzed and predicted by using online software.The results showed that amino acid sequences in 8 kinds of plants were similar,and α-helix was the largest component in 2-D structure.MDH was a chloroplast matrix protein in barley,and a cytoplasmic matrix protein in wheat,MDH in cassava and other plants was mitochondrial matrix protein with two functional domains.Compared with a cassava wild relatives M.esculenta ssp.flabellifolia (W14),The expressions of wild cassava M.glaziovii,M.esculenta SC205 and SC8 in storage roots were down regulated at the transcriptional level.These results would be helpful of further verify the biological functions of MDH in cassava.
NETL
NSTL
万方数据
维普
