Establishment of Female Core Collection of Eucommia ulmoides Oliv.Based on Phenotypic Characters
Female core collection was constructed based on measuring 27 quantitative traits from 395 female E.ulmoide germplasms.An optimal sampling strategy to construct the core collection was discussed,including genetic distance,sampling methods,sampling proportions and cluster selections.The advantages and disadvantages of each sampling.sampling strategies were tested by the value of MD,VD,CR and VR.The effective of core collection was detected by Shannon-weaver diversity index,accord rate and principal component analysis.The results showed that the optimal strategy was selected by combining the preferred method,euclidean distance,10%sampling proportion,and median distance clustering method.The rate ofMD,VD,CR,VR were 0,92.59%,100% and 147.28%,respectively.The final sampling proportion was 11.6%,and there was not significant correlation between the core collection and total collection by T-test.The coincidence rate between the core germplasm and the original germplasm on the 27 indicators was 96.05%~100%,The accord rate of maximum,minimum were 100%.The rate of diversity index was 85.78%~99.52%,Principal component analysis showed the top ten principal components from the core collection and total collection were identical,and the accumulative contribution rate were 81.431%,87.681%,respectively.The female core collection of 46 accessions could satisfactorily represented the phenotypic variation of 395 E.ulmoide germplasms.