Responses of Medicago sativa Seed to Different Salt Stresses During Germination
In the present study,the effects of salinity on seed germination ofMedicago sativa were evaluated by treated with six different types salt solution:NaCl,Na2SO4,KCl,K2SO4,CaCl2,NaHCO3.Germination rate,germination index,mean germination time,salt injury rate and relative inhibitory ratio of root length under different salts and different salt concentration have been investigated.The result showed that with increasing salt concentrations the germination rate and germination index of M.sativa seeds significantly decreased compared with the control.Meanwhile,the mean germination time,salt injury rate and relative inhibitory ratio of root length significantly increased compared with the control.The initial response salt concentration of M.sativa was:NaCl 25 mmol/L,K2SO4 50 mmol/L,CaCl2 100 mmol/L.The limiting salt solution concentration was:NaCl 190.97 mmol/L,Na2SO4 92.84 mmol/L,KCl 353.86 mmol/L,K2SO4 160.68 mmol/L,CaCl2 260.55 mmol/L,NaHCO3 114.77 mmol/L.The tolerance ofM.sativa seeds was different for the different salt type.The order of tolerance was:NaHCO3>Na2SO4 >K2SO4>NaCl >KCl>CaCl2.
Medicago sativaSalt stressSeed germinationGermination rateGermination index