首页|紫花苜蓿种子萌发过程中对不同盐胁迫的响应

紫花苜蓿种子萌发过程中对不同盐胁迫的响应

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本试验以6种盐溶液(NaCl,Na2SO4,KCl,K25O4,CaCl2,NaHCO3)对紫花苜蓿种子进行胁迫处理,研究不同盐胁迫下紫花苜蓿种子的发芽率、发芽指数、平均发芽时间、盐害率及根长抑制率的差异.结果显示,紫花苜蓿种子的发芽率、发芽指数随盐溶液浓度增加而降低;平均发芽时间、盐害率和根长抑制率随盐浓度的升高而增加.通过分析得出紫花苜蓿种子发芽过程中对不同盐胁迫的响应浓度分别为:NaCl 25 mmol/L,K2SO4 50 mmol/L,CaCl2 100 mmol/L;耐盐极限浓度分别为:NaCl 190.97 mmol/L,Na2SO4 92.84 mmol/L,KCl353.86 mmol/L,K2SO4 160.68 mmol/L,CaCl2 260.55 mmol/L,NaHCO3 114.77 mmol/L.紫花苜蓿种子萌发过程中对不同盐分胁迫的耐受性大小依次为:NaHCO3>Na2SO4 >K2SO4>NaCl>KCl>CaCl2.
Responses of Medicago sativa Seed to Different Salt Stresses During Germination
In the present study,the effects of salinity on seed germination ofMedicago sativa were evaluated by treated with six different types salt solution:NaCl,Na2SO4,KCl,K2SO4,CaCl2,NaHCO3.Germination rate,germination index,mean germination time,salt injury rate and relative inhibitory ratio of root length under different salts and different salt concentration have been investigated.The result showed that with increasing salt concentrations the germination rate and germination index of M.sativa seeds significantly decreased compared with the control.Meanwhile,the mean germination time,salt injury rate and relative inhibitory ratio of root length significantly increased compared with the control.The initial response salt concentration of M.sativa was:NaCl 25 mmol/L,K2SO4 50 mmol/L,CaCl2 100 mmol/L.The limiting salt solution concentration was:NaCl 190.97 mmol/L,Na2SO4 92.84 mmol/L,KCl 353.86 mmol/L,K2SO4 160.68 mmol/L,CaCl2 260.55 mmol/L,NaHCO3 114.77 mmol/L.The tolerance ofM.sativa seeds was different for the different salt type.The order of tolerance was:NaHCO3>Na2SO4 >K2SO4>NaCl >KCl>CaCl2.

Medicago sativaSalt stressSeed germinationGermination rateGermination index

王建科、王钧、胡延萍、李毅、柴文秀、王莉

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中国科学院西北高原生物研究所,西宁,810008

中国科学院大学,北京,100049

青海省互助县台子乡农村社会经济服务中心,海东,810500

紫花苜蓿 盐胁迫 种子萌发 发芽率 发芽指数

青海省科技计划项目国家自然科学基金

2014-NK-A4-2-131300269

2018

分子植物育种
海南省生物工程协会

分子植物育种

CSTPCDCSCD北大核心
影响因子:0.765
ISSN:1672-416X
年,卷(期):2018.16(1)
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