Development and Application of EST-SSR Molecular Marker for Lycium barbarum L.
In order to rapidly distinguish 8 different strains ofLycium barbarum,we collected 8 Ningxia Lycium barbarum to develop new SSR primers,and the results showed that these EST-SSR markers could be used to investigate and analyze the genetic structure ofL.barbarum.In addition,the SSR markers and clustering were used to show that the genetic polymorphisms of the tested materials were high,and 8 lines of Lycium barbarum had a high level of diversity loci,of which Ningqi 1 and Ningqi 3 had the highest genetic similarity.The similarity coefficient of Ningqi 8 and Ningqi 3 and 5 was 0.222 2,the similarity was the lowest and the phylogenetic relationship was far.Therefore,the 13 SSR primers could be used to identify the eight lines ofL.barbarum.For the identification and protection ofL.barbarum varieties,its scientific research might provide a scientific basis.
NETL
NSTL
万方数据
维普
