Knockout and Function Research of FGSG_08948 Gene in Fusarium graminearum
The purpose of this experiment was to knock out FGSG_08948 gene in Fusarium graminearum and to determine the phenotype of its deletion mutant, thereby analyzing the biological function of the gene. The FGSG_08948 gene was knocked out by Split-Marker method, and gene deletion cassette containing hygromycin phosphotransferase gene hph was constructed and was transformed into protoplast of wild type PH-1 by PEGmediated transformation method. Transformants were selected through medium containing hygromycin and then two deletion mutants were confirmed by PCR positive and negative screening, named as △FGSG_08948 1-1 and △FGSG_08948 1-2, respectively. The phenotype observation found that there was no significant difference between deletion mutant and wild type in colony morphology, spore morphology and pathogenicity. Colony growth rate of FGSG_08948 deletion mutant was slightly delayed. However, the spore morphology was not different, the pathogenicity was not weakened, but the sporulation increased. Therefore, FGSG_08948 gene might be related to spore formation in Fusarium graminearum.
NETL
NSTL
万方数据
维普
