甘菊[Chrysanthemum lavandulifolium(Fisch.ex Trautv.)Ling et Shih]为菊科菊属植物,二倍体,基因组较小,为栽培菊花的亲本之一.KRP是植物中独特的转录因子,参与细胞周期调控,影响植物生长和发育.本试验以甘菊为材料,克隆ClKRPs基因,分析其基本理化性质、构建系统进化树、miRNA靶基因预测、保守基序分析.结果表明,ClKRPs转录因子相对分子量为29.10～144.30,ClKRPs蛋白都为酸性蛋白;ClKRP1、ClKRP2、ClKRP3、ClKRP5、ClKRP6、ClKRP10、ClKRP12、ClKRP14、ClKRP15 蛋白为稳定蛋白,ClKRP4、ClKRP7、ClKRP8、ClKRP9、ClKRP11、ClKRP13、ClKRP16 为不稳定蛋白.系统发育分析显示,甘菊 ClKRPs蛋白可以分为两大类.利用MEME软件识别出6个甘菊ClKRP保守基序.生物信息学分析预测5个ClKRP基因与4个miRNA结合.本研究为进一步探索和分析甘菊ClKRPs转录因子的功能奠定了基础.
Identification and Molecular Biology Analysis of ClKRPs Transcription Factor in Chrysanthemum lavandulifolium
Chamomile[Chrysanthemum lavandulifolium(Fisch.ex Trautv.)Ling et Shih]is a plant of Chrysan-themum genus in the family of Asteraceae,which is diploid and has a small genome.It is one of the parents of cultivated chrysanthemums.KRP is a unique type of transcription factor in plants,which participates in cell cycle regulation and affects plant growth and development.In this experiment,chamomile was used as the material to clone the ClKRPs gene,analyze its basic physical and chemical properties,construct a phylogenetic tree,predict miRNA target genes,and analyze conservative motifs.The results showed that:ClKRPs transcription factor relative molecular weight was 29.10～144.30,ClKRPs proteins were acidic proteins;ClKRP1,ClKRP2,ClKRP3,ClKRP5,ClKRP6,ClKRP10,ClKRP12,ClKRP14,ClKRP15 proteins were stable proteins,ClKRP4,ClKRP7,ClKRP8,ClKRP9,ClKRP11,ClKRP13,ClKRP16 were unstable proteins.Phylogenetic analysis showed that the ClKRPs protein of chamomile could be divided into two categories.Six conserved motifs of camomile ClKRP were identified by MEME software.Bioinformatics analysis predicts that 5 ClKRP genes bound to 4 miRNAs.This study laid the foundation for further mining and analysis of the functions of the chamomile ClKRPs transcription factors.
NETL
NSTL
万方数据
维普
