Analysis of Interaction between Transcription Factors ZmBET10 and BTB-TAZ Protein in Maize(Zea mays)
In order to clarify the interaction between BTB-TAZ protein and transcription factor ZmBET10,the yeast two-hybrid vectors of BTB-TAZ protein ZmBT4,ZmBT2a,ZmBT2b and transcription factors ZmBET10(ZmBET10a and ZmBET10b)were constructed in this study.Yeast two-hybrid technology was used to study the interaction between BTB-TAZ protein and ZmBET10.The results showed that the yeast colonies that co-trans-formed with AD-ZmBT4,AD-ZmBT2a,AD-ZmBT2b combined with BD-ZmBET10a,BD-ZmBET10b could grow normally on three deficient medium(SD/-Leu/-Trp/-His)and three deficiency plus 3-AT(SD/-Leu/-Trp/-His/3-AT),while the control combinations could not grow normally,indicating that ZmBT4,ZmBT2a and ZmBT2b can di-rectly interact with ZmBET10 in yeast cells.In order to clarify the function of BTB domain in the interaction be-tween BTB-TAZ protein and transcription factor ZmBET10,yeast two hybrid vectors of BTB domain of ZmBT4,and ZmBT2b proteins were constructed.The interaction between BTB domain and transcription factor ZmBET10 was further studied by yeast two hybrid technique.The results showed that BTB domain of ZmBT4 and ZmBT2b proteins could interact with ZmBET10,indicating that BTB domain plays an important role in the interaction be-tween BTB-TAZ protein and transcription factor ZmBET10.These results laid a foundation for further study on the function and mechanism of BTB-TAZ protein in maize disease resistance.
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万方数据
维普
