Cloning and Expression Analysis of Soluble Inorganic Pyrophosphatase Gene from Sorghum
Soluble inorganic pyrophosphatase in plants can play an important regulatory role in the conversion of sugar and starch by coordinating the sugar production reaction in the cytoplasm and the glycolysis reaction in the plastid.In order to clarify the function of SbPPase gene in the regulation of sorghum,in this study,the gene from'Tx623B'was cloned and analyzed by bioinformatics and RT-qPCR.The results showed that the total length of SbP-Pase gene was 1 217 bp,which contained 6 exons,5 introns,CDS of 645 bp and encodes 214 amino acids.It is a hydrophilic protein with high stability.Conserved domain analysis showed that it contained a typical pyrophospha-tase domain,which belong to c100217 superfamily.The phylogenetic tree showed a close relationship with maize and the highest homology in Gramineous millet crops.According to RT-qPCR analysis,the expression level of SbP-Pase gene in leaves was low and stable.The expression level of the gene in root gradually increased as sorghum matures,which speculated that SbPPase gene was closely related to stress.It is provides a theoretical basis for ex-ploring the path of sugar synthesis in sorghum and improving sugar content traits in the study of the sorghum.
NETL
NSTL
万方数据
维普
