Transcriptome Analysis of Dengmu Rape(Brassica campestris)under Drought Stress
In order to explore the gene expression of Brassica campestris L.under drought stress,Dengmu rape was used as the test material,normal irrigation at seedling stage as the control,and drought for 8 days as the treatment group.By analyzing the differentially expressed genes under different water conditions in Dengmu rape,we found that there were 3 057 significantly different genes under different water conditions,including 1 616 significantly up-regulated genes and 1 441 significantly down regulated genes.There were 7 172 up-regulated genes and 731 down regulated genes in GO annotation;through the enrichment analysis of KEGG pathway,we found that RNA degradation,eukaryotic ribosome biosynthesis,ribosome,pyrimidine metabolism and purine metabolism were the main pathways involved in the enrichment of up-regulated differential genes under drought stress.The main path-ways involved in the down-regulation of differential gene enrichment under drought stress were valine,leucine and isoleucine degradation,plant hormone signal transduction,photosynthesis,linoleic acid,α-linolenic acid metabolism,etc.;a total of285 555 transformed SNPs and 485 985 transversed SNPs were found.These results indi-cated that there were differences in gene expression of Dengmu rape under drought stress,which enriched the gene information database of Brassica campestris L.and laid a foundation for molecular breeding in later stage.
NETL
NSTL
万方数据
维普
