Transcriptome sequencing was performed on biennial flower buds from male sterile lines(MS 137,MS301)and maintainer lines(OT154,OT302)of sugar beet.A total of257 differentially expressed genes at flower bud stage were obtained by analysis.GO functional annotation showed that differentially expressed genes were mainly concentrated in catalytic activity,hydrolytic activity,carbohydrate metabolism and transport in biological processes.KEGG functional annotation indicated that the differentially expressed genes were involved in metabolism,pentose and glucuronic acid conversion,and starch and sucrose metabolism.Based on GO and KEGG analysis of differential expression,8 differentially expressed genes were selected,including SGNH hydrolase gene,GDSL esterase gene,galacturonase gene,pectin lyase gene,pectin esterase gene and glycoside hydrolase,which were all up-regulated in CMS lines.UDP-glucosyltransferase gene and cytochrome P450 gene were up-regulated in maintainer lines.The results provide a theoretical basis for further exploring the mechanism of fertility regula-tion of male sterile lines in sugar beet.
NETL
NSTL
维普
万方数据
