Gene Cloning and Protein Prokaryotic Expression of RZFP Zinc Finger Protein in Cassava
Zinc finger proteins play an important role in plant growth and development and adaptation to enviro-nmental stress.In order to better study the function of zinc finger protein in cassava growth and development and resistance to stress.In this study,a MeRZFP gene was isolated,identified and cloned.The sequencing results showed that the gene size was 1 068 bp.Firstly,the study analyzed its phylogenetic tree and conservative domain,and found that it contains two conservative domains:zinc-ribbon and zinc-finger-C3C4.The prokaryotic expression vector pET28a-MeRZFP was further constructed,and the temperature that was relatively suitable for the growth of the strain was screened.The results showed that the optimal expression temperature of the protein was 28 ℃.The recombinant protein pET28a-MeRZFP was detected by SDS-PAGE electrophoresis and verified by Western blot.The results showed that the size of the protein was 39 kD.It provides a reference for further exploring the function of cassava RZFP in cassava's response to environmental stress.
NETL
NSTL
万方数据
维普
