Genome-wide Identification and Comparative Evolutionary Analysis of the KEA Gene Family in Five Brassicaceae Plant Species
K+efflux antiporter plays an important role in intracellular K+and pH homeostasis,and function in plant nutrition,growth,development and signal transduction.In this study,genome-wide analysis identified 6,6,12,18 and 6 KEA genes in five Brassicaceae plants,Arabidopsis thaliana,Arabidopsis lyrate,Arabidopsis pumila,Camelina sativa and Eutrema salsugineum,respectively.Phylogenetic evolution,gene structure,and motif analyses showed that 48 KEA genes were classified into Clade Ⅰ and Clade Ⅱ subfamilies.Collinearity analysis revealed that during the evolution of A.pumila and C.sativa,the KEA family members experienced both whole-genome duplication and segmental duplication events,while most of the KEA members in A.thaliana,A.lyrata and E.sal-sugineum were mainly dispersed duplication events.Comparative evolutionary analysis demonstrated that purifica-tion selection was the main driving force for the evolution of the KEA paralogous genes in five plants.Collinearity analysis of KE A genes among species indicated a complex evolutionary relationship between Brassicaceae species.The expression of ApKEA genes significantly respond to high salt stress.In addition,the expression patterns of ApKEA2.1/2.2 and ApKEA6.1/6.2 were significantly different,indicating that they functionally diverged.The re-sults of this study expanded our understanding of the K+/H+antiporters in plants,and provided genetic resources and theoretical bases for further analysis of K+/H+balance and potassium dynamic nutrition mechanism.
NETL
NSTL
万方数据
维普
