摘要
K+/H+逆向转运蛋白通过调节细胞内K+和pH稳态,在植物的营养、生长、发育和信号转导中发挥重要作用.在本研究中,利用全基因组分析5种十字花科植物拟南芥(Arabidopsis thaliana)、琴叶拟南芥(Ara-bidopsis lyraia)、小鼠耳芥(Arabidopsis pumila)、亚麻荠(Camelina sativa)和盐芥(Eutrema salsugineum)分别鉴定出6、6、12、18和6个KEA基因.系统进化、基因结构和motif分析表明以上48个KEA基因隶属于Clade Ⅰ和Clade Ⅱ两个亚家族.共线性分析发现,在小鼠耳芥和亚麻荠进化中,KEA家族成员既经历了全基因组复制同时又经历了片段重复事件,而拟南芥、琴叶拟南芥和盐芥中大部分KEA成员以经历分散型复制事件为主.比较进化分析表明5种植物KEA家族的旁系同源基因在进化中的主要动力为纯化选择.物种间KEA基因的共线性分析表明十字花科物种之间存在复杂的进化关系.小鼠耳芥KEA基因的表达明显响应高盐胁迫,其中ApKEA2.1/2.2、ApKEA6.1/6.2的表达模式存在明显差异,暗示复制基因对功能发生分歧.本研究是对植物中K+/H+逆向转运蛋白的新认识,研究结果为进一步研究K+/H+平衡与钾素动态营养机制提供了基因资源和理论基础.
Abstract
K+efflux antiporter plays an important role in intracellular K+and pH homeostasis,and function in plant nutrition,growth,development and signal transduction.In this study,genome-wide analysis identified 6,6,12,18 and 6 KEA genes in five Brassicaceae plants,Arabidopsis thaliana,Arabidopsis lyrate,Arabidopsis pumila,Camelina sativa and Eutrema salsugineum,respectively.Phylogenetic evolution,gene structure,and motif analyses showed that 48 KEA genes were classified into Clade Ⅰ and Clade Ⅱ subfamilies.Collinearity analysis revealed that during the evolution of A.pumila and C.sativa,the KEA family members experienced both whole-genome duplication and segmental duplication events,while most of the KEA members in A.thaliana,A.lyrata and E.sal-sugineum were mainly dispersed duplication events.Comparative evolutionary analysis demonstrated that purifica-tion selection was the main driving force for the evolution of the KEA paralogous genes in five plants.Collinearity analysis of KE A genes among species indicated a complex evolutionary relationship between Brassicaceae species.The expression of ApKEA genes significantly respond to high salt stress.In addition,the expression patterns of ApKEA2.1/2.2 and ApKEA6.1/6.2 were significantly different,indicating that they functionally diverged.The re-sults of this study expanded our understanding of the K+/H+antiporters in plants,and provided genetic resources and theoretical bases for further analysis of K+/H+balance and potassium dynamic nutrition mechanism.
基金项目
国家自然科学基金(U1303302)
石河子大学国际合作项目(GJHZ201806)
安徽科技学院学科带头人引进人才启动经费项目(NXYJ202001)
NETL
NSTL
维普
万方数据