Bioinformatics Analysis of DlFNS Protein in Dimocarpus longan Lour.
The aim of this study is to analyze the structure of the DlFNS protein identified from Dimocarpus lon-gan and its biological function.In this study,a flavone synthase(FNS)gene was screened and identified from D.lon-gan RNA-seq data(SRA accession:SRP155595).Multiple bioinformatics approaches were adopted to systemati-cally analyze the DlFNS gene.The results showed that the DlFNS protein translated from the DlFNS gene contains 530 amino acids,with its molecular formula being C2731H4353N735O764S21.It has a transmembrane structure but does not contain signal peptides.It was predicted to be a hydrophilic protein locating in endoplasmic reticulum.The secondary structure of DlFNS protein was analyzed,suggesting that DlFNS protein was mainly composed of ran-dom coil and α-helix.According to the evaluation by Ramachandran,the tertiary structure of DlFNS protein con-structed by SWISS-MODEL was reliable.The results of phylogenetic tree indicated that the DlFNS protein has a close relationship with a FNS protein identified from Pistacia vera L.The results of this study will facilitate the fur-ther study on the function of DlFNS gene and lay a basis for elucidating its role in terms of mediating the biosyn-thesis of flavonoid.
NETL
NSTL
万方数据
维普
