RNA-Seq Mining Differentially Expressed Genes of Riboflavin Synthesis Pathway in Armillaria luteo-virens
To exploring the differences in the gene expression of Armillaria luteo-virens and Agaric us bisporus,and excavation of major genes in riboflavin synthesis pathway,RNA_seq was used to sequence the transcriptome of A.luteo-virens and A.bisporus.There were 26 818 unigenes significantly differentially expressed,including 11 236 up-regulated and 15 582 down-regulated.KEGG enrichment results showed that 19 988 genes were in-volved in the plant secondary metabolites synthesis,which were 813 differentially expressed genes in vitamin metabolism pathway.In the riboflavin pathway,five structural genes involved in riboflavin biosynthesis were not differentially expressed between A.luteo-virens and A.bisporus,and six genes were up-regulated in A.lu-teo-virens.The homologous gene CL911.Contig3_ALL(Almch5)of MCH5,a riboflavin transporter,was specifi-cally expressed in A.luteo-virens,and the log2FC value was 12.98.Meanwhile,Almch5 was highly homologous to the riboflavin transporter MCH5 in Hypsizygus marmoreus,and has a similar functional domain of 7bp3.1A,which proves that Almch5 is likely to be the main effector protein of riboflavin in A.luteo-virens,which provide new clues for further study of riboflavin synthesis mechanism.
NETL
NSTL
万方数据
维普
