Molecular Identification of Chinaberry Yellows Phytoplasma
In order to clarify the taxonomic status of phytoplasmas associated with yellowing leaf of chinaberry in Hainan.The study,cloned the 16S rDNA gene and rp gene of chinaberry yellows phytoplasma,and performed consistency analysis of gene sequences,phylogenetic analysis,and virtual RFLP analysis of 16S rDNA gene.The results showed that the size of the cloned 16S rDNA gene segment was 1 830 bp,and the size of the rp gene seg-ment was 1 212 bp.The 16S rDNA gene fragment of the chinaberry yellows phytoplasma had the highest homolo-gy of 100％with the phytoplasma of 16SrI group,and the rp gene fragment of the chinaberry yellows phytoplasma had the highest homology of 100％with the phytoplasma of rpI group.Phylogenetic tree of 16S rDNA gene showed that the chinaberry yellows phytoplasma in the study were clustered into one clade with the arecanut yel-low leaf disease phytoplasma(KF728948.1),'Oenothera'phytoplasma(M30790.1);the phylogenetic tree of rp gene showed that the chinaberry yellows phytoplasma and aster yellows phytoplasma(AY183708.1)clustered in the same independent clade.The virtual RFLP map of 16S rDNA gene of chinaberry yellows phytoplasma was the same as that of onion yellows phytoplasma(AP006628)which is a represented strain by 16SrI-B subgroup,and the similarity coefficient was 1.00.In summary,Hainan lines of chinaberry yellows phytoplasma belonged to 16SrI-B subgroup in classification.The results of this study can provide a theoretical basis for the prevention and control of areca palm yellow disease by eradicating its intermediate host.
NETL
NSTL
万方数据
维普
