Comparison of Common Detection Methods of Sugar Beet Ploidy
Understanding the ploidy of germplasm resources and breeding materials is the basis of sugar beet breeding.Screening simple and reliable methods for ploidy detection is helpful to improve the identification effi-ciency of sugar beet germplasm resources.In this study,the ploidy of four sugar beet cultivars was detected by means of root tip chromosome,leaf stomatal guard cell chloroplast count and flow cytometry,which are three commonly used methods.The results showed that the chromosomes in the prophase of cell division were more eas-ily observed by using sugar beet root tips.There were 18 diploid chromosomes and 36 tetraploid chromosomes,the average number of chloroplasts in stomatal guard cells was 16.13 in diploid and 22 in tetraploid,the average peak value of G0/G1 for diploid materials was 305 796.53,and the average value of tetraploid materials was 656 593.78.The results of chromosome observation and flow cytometry were more reliable,and the chloroplast counting method of leaf stomatal guard cells was easily affected by material,external environment and operator,which af-fected the determination of material ploidy.Compared with chromosome observation,which is costly,difficult to operate and specialized,the chloroplast counting and flow cytometry of stomatal guard cells in leaves are simple,rapid and operable,and can be given priority in the screening of a large number of materials.In this study,the ad-vantages and disadvantages of the three ploidy detection methods were analyzed and compared through experi-ments,so as to provide a reliable method for the identification and utilization of sugar beet breeding resources.
NETL
NSTL
万方数据
维普
