烟草酪氨酸脱氢酶NtTYRA1影响主流烟气中苯酚成分的释放
Tobacco Arogenate Dehydrogenase NtTYRA1 Affects the Release of Phenol in Mainstream Cigarette Smoke
李正风 1王燃 2金立锋 2李锋 2魏攀 2孙涛 2王大伟3
作者信息
- 1. 云南中烟工业有限责任公司,昆明,650231
- 2. 中国烟草总公司郑州烟草研究院国家烟草基因研究中心,郑州,450001
- 3. 中国农业科学院烟草研究所,烟草行业烟草基因资源利用重点实验室,青岛,266101
- 折叠
摘要
酪氨酸是烟气苯酚的首要前体物质之一.酪氨酸脱氢酶(TYRA/ADH)在质体中催化阿罗酸形成酪氨酸.本研究通过TILLING方法在EMS诱变群体中筛选鉴定了 tyrA1突变体,明确了其突变类型,测定了部分突变体的酪氨酸等氨基酸含量,以及卷烟烟气中苯酚等酚类物质释放量.结果表明,鉴定到的3个tyrA1突变功能有害突变体中,NtTYRA1蛋白关键活性部位的三级结构由于氨基酸变异发生变化,影响了其催化功能.NtTM01和NtTM05 2个突变体的酪氨酸含量较野生型对照分别下降67.44％和85.15％,而NtTM01突变体卷烟样品的主流烟气苯酚释放量极显著降低了 11.89％,其他酚类物质含量也显著或极显著降低.本研究初步认为,NtTYRA1基因的突变可以有效降低酪氨酸含量,进而降低卷烟主流烟气中苯酚的释放量,可以将其作为基因工程靶标基因,用于培育低烟气苯酚释放的烤烟新品种.
Abstract
Tyrosine is one of the precursors of phenol in cigarette smoke,and biosynthesized by tyrosine dehydro-genase/arogenate dehydrogenase(TYRA/ADH)catalyzing arroic acid in plastids.In this study,tyrA1 mutants were screened and identified in EMS mutagenesis population of tobacco(Nicotiana tabacum L.)by TILLING method,and their mutation types were defined.The contents of tyrosine and other amino acids in some mutants,as well as the release of phenol and other phenolic substances in cigarette smoke were determined.The results showed that tertiary structures of the key active site in NtTYRA1 protein were changed due to amino acids variation,which affected the catalytic function of the three tyrA 1 functionally deleterious mutants identified.The contents of tyrosine in NtTM01 and NtTM05 mutants were 67.44％and 85.15％lower than those in the wild type control,respectively.Moreover,the release of phenol in the mainstream smoke of NtTM01 mutant was significantly decreased by 11.89％,and the contents of other phenolic substances were also significantly or extremely significantly decreased.These results showed that the mutation of NtTYRA 1 gene can effectively reduce the content of tyrosine,and subsequently reduce the release of phenol in mainstream cigarette smoke.It can be used as a target gene in genetic engineering to cultivate new varieties of flue-cured tobacco with low phenol release in cigarette smoke.
关键词
烟草突变体/酪氨酸脱氢酶/酪氨酸/苯酚释放/卷烟烟气Key words
Tobacco mutant/Arogenate dehydrogenase/Tyrosine/Phenol release/Cigarette smoke引用本文复制引用
基金项目
国家烟草专卖局烟草基因组计划重大专项(11201901019[JY-06])
出版年
2024
NETL
NSTL
维普
万方数据