Establishment of Rapid Propagation Systems for Zhongzhe Sugarcane Cultivars
In order to popularize Zhongzhe sugarcane cultivars,this research obtained the method of tissue culture seedlings of'Zhongzhe1'(ZZ1),'Zhongzhe6'(ZZ6)and'Zhongzhe9'(ZZ9)with high smut resistance by axillary bud culture.The results showed that for ZZ1,the optimum medium for cluster bud induction and tissue culture seedling proliferation was MS containing 6-BA 2.0 mg/L and KT 0.5 mg/L,the optimum rooting medium was 1/2MS containing NAA 1.0 mg/L.For ZZ6,the optimum medium for cluster bud induction and tissue culture seedling proliferation was MS containing 6-BA 2.0 mg/L and KT 0.5 mg/L,the optimum rooting medium was 1/2MS containing NAA 3.0 mg/L.For ZZ9,the optimum medium for cluster bud induction was MS containing 6-BA 2.0 mg/L,the optimum medium for tissue culture seedling proliferation was MS containing 6-BA 1.0 mg/L,the optimum rooting medium was 1/2MS containing NAA 2.0 mg/L.The research provides some reference for promoting the cultivation and popularization of healthy seedlings of Zhongzhe sugarcane cultivars.
SugarcaneZhongzhe cultivarsTissue culture and rapid propagationAxillary bud culture