Identification and Bioinformatics Analysis of ARF Gene Family in Walnut(Juglans regia L.)
Auxin response factor(ARF)is a type of transcriptional regulatory factor,which plays a key role in the process of plant growth and development.Based on the genomic database of walnut(Juglans reiga L.).In this study,we used bioinformatics methods to identify the members of the ARF family in walnut via HMMER 3.0 and BLAST software at the genome-wide level.A total of 30 walnut ARF genes were identified,and the encoded protein ranged in length from 596 to 1 124 aa,the molecular weight of the protein were between 65 447.51 and 125 471.08 Da,the isoelectric point were 5.27～8.31,and all genes are located in the nucleus.According to the phylogenetic tree,these genes were classified into 4 subgroup(ClassⅠ～ClassⅣ),members of the same subgroup usually showed similar gene structures and conserved domains.Conserved domain analysis showed that all 30 JrARF proteins have conserved B3.Aux_resp domains,and the most of ARF family members contain Aux/IAA domains.These genes are unevenly distributed on the 15 chromosomes of walnut.Cis-acting elements indicated that the members of this family were related to many light response elements and stress reponse,hormone response and other response elements.Transcriptome data was used to analyze the expression patterns of ARF family mem-bers during the differentiation of female and male flower buds in walnut.The results showed that JrARF1,JrARF5,JrARF17 were highly expressed during the initial differentiation stage of female flower buds(FB-2).JrARF6 and JrARF20 were highly expressed in male flower buds(MB-3).It provides an important theoretical basis of the mechanism of the ARF gene family in walnut.
NETL
NSTL
万方数据
维普
