Identification,Sequence Analysis and Evolutionary Inference of Plant Inver-tase Family Genes
Invertase CWIN(Cell wall invertase),VIN(Vacuolar invertase)and CIN(Cytoplasmic invertases)is the key enzyme in sucrose metabolic pathway to convert sucrose to glucose and fructose,which is directly in-volved in the biosynthesis of plant sucrose metabolism.In order to systematically comb the status of invertase re-lated genes in plant genomes,laboratory carried out a comprehensive identification and evolutionary analysis of its genes and focused on the plant expression in Arabidopsis thaliana and Solanum lycopersicum.Firstly,the laborato-ry identified the conserved domain of CWIN,VIN and CIN gene sequences,and on this basis comprehensively iden-tified related genes and constructed their evolutionary tree.Secondly,A rabidopsis thaliana and Solanum lycoper-sicum CDS sequences were aligned to each probe to extract tissue expression data from the database.Finally,through domain identification,evolutionary tree analysis and expression analysis,the biosynthesis mechanism of CWIN,VIN and CIN in plants was obtained.This study provides genetic information for invertase related genes and is helpful to study the mechanism of biosynthesis pathway of sucrose metabolism and the evolutionary mecha-nism of genes related to sucrose metabolism in plants.
NETL
NSTL
万方数据
维普
