Progress on Molecular Authentication of Bletilla striata
Bletilla striata is a traditional Chinese herb,which has a very high medical value.Recently,with B.striata price increase,Chinese traditional medicine market is flooded with a lot of its adulterants.Compared with the traditional morphological,physical and chemical and microscopical identification,molecular identification is faster,more accurate and objective.The molecular identification method is based on the difference of molecular characteristics between organisms,which is not affected by external factors such as natural environment,growth and development stage etc..Here,we reviewed two authenticating strategies of B.striata based on the properties of molecular markers and DNA barcoding.The molecular markers can identify the affinity of B.striata varieties,mainly involving ISSR,SCAR,SSR,SNP and other markers.The ITS2 sequence combined with the secondary structure analysis and ITS+ycfl sequence could identify the Bletilla genus and adulterants.This literature review can provide some useful reference for the further study of the molecular identification methods of B.striata.
NETL
NSTL
万方数据
维普
