Analysis on Leaf Color and Yield Traits from Segregation Populations of Iron-deficient Mutants in Adzuki Bean
In order to identify the variation of leaf color character and its effect on yield in the mapping popula-tion of iron absorption and metabolism mutants of adzuki bean.In this study,leaf color segregation was in-vestigated in F2 populations of'Hebei801'(iron deficiency tolerant wild type)×FMY011(iron deficiency sensitive mutant)and MY076(iron deficiency tolerant mutant)×GM352(iron deficiency sensitive resource).The chloro-phyll SPAD values were determined and the correlations between leaf color separation and yield per plant and other agronomic traits were analyzed.The difference of chlorophyll SPAD value between new leaf yellowing and new leaf green individuals in F2 segregation population was extremely significant.Correlation analysis showed that SPAD value was significantly positive correlation with yield per plant,total number of pod per plant and hundred grain weight.The new leaf yellowing caused by iron deficiency would reduce the yield of adzuki bean.The phenotype segregation of leaf color was verified under iron deficiency stress.The results showed that the leaf color phenotype of HB801×FMY011 population was highly consistent with the leaf color segregation in the field,the F2∶3 lines from F2 new leaf yellowing individuals all displayed new leaf yellowing.The 96.62％F2:3 lines from new leaf green individuals in F2 exhibited new leaf green or heterozygous leaf color segregation under iron deficiency stress.Only the 3.38％F2∶3 lines from F2 new leaf green individuals showed the different new leaf yellowing phenotype.In MY076×GM352 segregation population,the field normal leaf color phenotype was consistent with that under iron deficiency stress,but only the 36.07％F2∶3 lines from F2 new leaf yellowing individuals still were new leaf yellowing under iron deficiency stress,and 60.66％F2∶3 lines from the new leaf yellowing individuals in F2 showed heterozygous leaf color separation under iron deficiency stress.3.28％F2∶3 lines from the new leaf yellowing individuals in F2 exhibited the different new leaf green phenotype.The leaf color investigation of segregation population in field may preliminarily estimate the leaf color segregation of both mutant populations,but accurate phenotypic identification still needs to be investigated under iron deficiency stress.This study provided basic populations and phenotype data for further gene mapping and cloning related to iron absorption and metabolism,and for iron bioenhancement breeding in adzuki bean.
Adzuki bean(Vigna angularis L.)Leaf color segregation populationsIron deficiencyCorrelation analysis
NETL
NSTL
万方数据
维普
