Identification of Pathogen Species of Root Knot Nematode on Tomato in Chongqing
In order to identify the species of root knot nematodes endangering tomato in Chongqing,root samples of tomato root knot nematodes were collected by 5-point sampling method from major vegetable bases in Tongnan District,Bishan District,Jiulongpo District,Banan District,Hechuan District and Jiangjin District in Chongqing in 2020.Root knot nematodes were isolated and identified by morphological and molecular biological identification methods.The results showed that the female adults of root knot nematode were white lemon shaped,the perineal pattern of female adult was nearly circular,the line grain were wavy,there was no obvious side line,the back arch was high,trapezoidal,and the top was smooth,these characteristics were similar to the morphological characteris-tics of Meloidogyne incognita reported;this study found only the specific primers of Meloidogyne incognita could amplify the target fragment.The sequencing results of the amplified target fragment were compared by blastn.The result showed that the consistency between the fragment and a cDNA sequence(Sequence ID:JK286281.1)of Meloidogyne incognita was 93.75％.Combining with the identification results of morphology and molecular biolo-gy,the species of root knot nematode endangering tomato in Chongqing was Meloidogyne incognita.The results of this study indicated great effect on contolling of root knot nematode disease and breeding for resistance to the di-sease on tomato in Chongqing.
NETL
NSTL
万方数据
维普
