Screening of Kumquat Germplasm with Specific SSR Markers and Analysis of Genetic Relationship
In order to screen the specific SSR markers and solve the problem of synonym and homonym of kumquat plants.In this study,38 samples of kumquat and its related genera were selected and amplified by 23 pairs of primers to obtain 265 effective bands,250 polymorphic bands,and the polymorpnism ratio was 92.99％.The amplifiled fragment was from 100 to 1 200 bp.SSR marker screening resulted in 47 specific markers.The num-ber of specific markers differed greatly between different germplasms.The most Gongsongju reached 7 specific markers.Citrus and Poncirus germplasms could be labeled without specificity.The 11 germplasms identified by the marker are are all of the Fortunella.Cluster analysis found that the genetic similarity coefficients of Fortunella,Citrus and Poncirus are all above 0.70,which indicates that Fortunella are closely related to plants of Citrus and Poncirus.At the similarity coefficient of 0.902,the 11 germplasms with no specific markers were clustered together,which also showed that the phylogenetic realtionship of the kumquat cultivars was close,and there existed the syn-onym and homonym.
NETL
NSTL
万方数据
维普
