Ameliorative Effects of Leonurine,the Active Alkaloid from Leonurus japonicus,on Exercise-Induced Fatigue in Rats
This study explored the alleviative effects of leonurine,an active alkaloid from Leonurus japonicus(Chinese motherwort),on exercise-induced fatigue(EIF)in rats and its mechanism in regulating the Nrf2/Keap1/ARE signaling pathway.Using animal models,the experiment investigated the protective role of leonurine on skeletal muscles and its functions in modulating antioxidative enzyme activity and reducing cellular oxidative stress.The results revealed that leonurine significantly extended the exhaustive swimming time of rats,reduced serum levels of LDH,CK and cTnI,and notably increased the activity of SOD,CAT and GSH-Px in skeletal muscles,thereby reducing MDA levels.These findings unveiled the significant role of leonurine in mitigating exercise-induced fatigue.Further research discovered that leonurine activates the Nrf2/Keap1/ARE signaling pathway,enhancing the expression of Nrf2 and its downstream target genes HO-1 and NQO-1,while suppressing the expression of Keap1,providing stronger antioxidative protection to skeletal muscles.When the Nrf2 inhibitor ML385 was used,the effect of leonurine was inhibited,verifying its mechanism of action through the Nrf2/Keap1/ARE signaling pathway.The outcomes of this research indicate that leonurine,as a natural active alkaloid,has significant anti-EIF effects and reveals its substantial potential as a novel natural compound for anti-fatigue through modulating the Nrf2/Keap1/ARE signaling pathway to alleviate oxidative damage in skeletal muscles.
NETL
NSTL
万方数据
维普
