Cloning and Expression Analysis of GhMA PKKKK3 Gene in Gossypium hirsutum L.
Mitogen-activated protein kinase(MAPK)plays an important regulatory role in plant growth,develop-ment,stress resistance,disease resistance and hormone signal transduction.In this study,the GhMA PKKKK3 gene was cloned using the cDNA of Gossypium hirsutum as a template.Its open reading frame is 1 998 bp and encodes 665 amino acid residues.The expression patterns of GhMA PKKKK3 gene under different stress induction condi-tions and different cotton varieties were analyzed by RT-qPCR.The results showed that the GhMA PKKKK3 gene had a certain degree of response to high salt,drought,low temperature and Verticillium dahliae treatment,and the gene was regulated most strongly under salt stress,followed by drought stress and cold stress;when induced by V.dahliae,it was only significantly higher than the control at about 1 h.The expression of this gene in the root tissues of different varieties of cotton was lower than that of the stem and leaf tissues.GhMA PKKKK3 gene expression dif-fered among varieties with different Verticillium wilt resistance but there was no significant pattern.The results of this study provide important clues for the screening of cotton resistance-related genes and further study of the func-tion of GhMA PKKKK3 gene.
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