分子植物育种2024,Vol.22Issue(3) :704-717.DOI:10.13271/j.mpb.022.000704

马铃薯全基因组StbZIP基因家族的鉴定及在不同逆境中的表达分析

Genome-wide Identification of bZIP Gene Family in Potato and Their Ex-pression Analysis under Different Stress

水清明 李海珀 韩儆仁 权小兵 秦天元 李亚杰
分子植物育种2024,Vol.22Issue(3) :704-717.DOI:10.13271/j.mpb.022.000704

马铃薯全基因组StbZIP基因家族的鉴定及在不同逆境中的表达分析

Genome-wide Identification of bZIP Gene Family in Potato and Their Ex-pression Analysis under Different Stress

水清明 1李海珀 2韩儆仁 1权小兵 1秦天元 3李亚杰1
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作者信息

  • 1. 定西市农业科学研究院,定西,743000
  • 2. 定西市种子站,定西,743000
  • 3. 甘肃农业大学农学院,兰州,730070
  • 折叠

摘要

碱性亮氨酸拉链(basic leucine zipper,bZIP)是一种广泛存在于植物中的保守转录因子,其家族成员数目是植物中最丰富的转录因子家族之一.为了解马铃薯StbZIP基因家族的相关信息,本研究通过生物信息学的方法,在二倍体马铃薯'DM'全基因组中鉴定64个马铃薯StbZIP家族成员,命名为StbZIP1~StbZIP64.通过分析发现,马铃薯StbZIP家族成员不均匀地分布在12条染色体上.生物信息学分析表明,家族成员蛋白长度为132~822aa,分子量介于15.40~88.18 kD之间,等电点4.74~10.30.系统发育树分析显示,该基因家族可分为11个亚群,其基因主要分布在第1、4和10号染色体上.基因结构和保守基序分析发现,各亚族成员间具有相似的保守基序和进化的保守性.表达模式分析发现,11个亚族内部呈现出响应不同非生物胁迫的表达模式,其中有8个亚族基因都在ABA胁迫24 h时表达量发生显著升高.这些结果为阐明StbZIP基因家族的进化和进一步研究StbZIP基因的功能提供了理论指导.

Abstract

Basic leucine zipper(bZIP)is a conserved transcription factor widely existing in plants.Its family mem-bers are one of the most abundant transcription factor families in plants.In order to understand the related informa-tion of potato StbZIP gene family,64 members of potato StbZIP family were identified in the whole genome of diploid potato'DM'by bioinformatics method,and named StbZIP1~StbZIP64.The results showed that the StbZIP gene were unevenly distributed on 12 chromosomes.The length of StbZIP family members was between 132 to 822 aa,the molecular weight was between 15.40 to 88.18 kD,and the isoelectric point was between 4.74 to 10.30.Phylogenetic tree analysis showed that the StbZIP genes were divided into 11 subfamilies,and its genes were main-ly distributed on chromosome 1,4 and 10.Analysis of gene structure and conserved motifs showed that members of each subfamily had similar conserved motifs and evolutionary conserved nature.Analysis of expression patterns revealed that 11 subgroups showed expression patterns in response to different abiotic stresses.Among them,8 subgroup genes showed a significant increase in expression levels at 24 h of ABA stress.These findings provide useful information to elucidate the StbZIP gene family's evolutionary relationship and to further analyze the func-tional characteristics of the StbZIP gene.

关键词

马铃薯/StbZIP基因家族/比较基因组/基因表达

Key words

Potato/StbZIP gene family/Comparative genome/Gene expression

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基金项目

甘肃省重大科技专项(20ZD7NA007)

出版年

2024
分子植物育种
海南省生物工程协会

分子植物育种

CSTPCD北大核心
影响因子:0.765
ISSN:1672-416X
参考文献量44
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