Bioinformatics Analysis of the Transcriptome of Young Leaf of Vaccinium ashei'Gardenblue'
To obtain the genetic information of Vaccinium ashei'Gardenblue',the transcriptome of the young leaves of'Gardenblue'cultivated in Guizhou was sequenced by high-throughput sequencing technology.Bioinfor-matics analysis was carried out on the sequencing data.About 1.4 Gb of pure data were obtained,and 32 093 uni-genes were assembled.The unigenes annotated in GO database are related to totals of 48 physiological and metabolic functions related to biological processes,cell components and molecular functions.27 137 unigenes could be annotated by KOG database,involving 25 metabolic pathways.9 525 unigenes could be annotated success-fully in KEGG database,involving 5 functional classes,19 functional mesoclasses and 128 metabolic pathways.25 418 unigenes could be annotated by NR database.18 309 unigenes could be annotated by SwissProt database.A total of 25 447 unigenes were annotated from the above four databases,accounting for 79.29％of the total uni-genes.8 846 unigenes were annotated by the above four databases,accounting for 27.56％of all unigenes.At the same time,bioinformatics analysis also showed that there were 758 unigenes encoding transcription factors,in-volving 51 families.There were 2 085 unigenes coding resistance genes,which involved 17 families.A total of 4827 SSR loci were detected,including 3 165 two-base repeats,accounting for 65.57％of the total number of SSR loci,followed by 1 431 trinucleotides,accounting for 29.65％.There were 231 tetranucleotides,pentanucleotides and hexanucleotides,accounting for 4.79％.The results have a strong reference value for understanding the growth and development,physiological and biochemical mechanisms of Vaccinium ashei.
万方数据
