分子植物育种2024,Vol.22Issue(3) :751-760.DOI:10.13271/j.mpb.022.000751

基于转录组数据的绢毛委陵菜MYB转录因子家族分析

Analysis of the MYB Transcription Factor Family of Potentilla sericata Based on Transcriptome Data

刘振 武俊刚 李响 夏威 秦建 刘庆鹏 王学刚 范卫芳 吴建慧
分子植物育种2024,Vol.22Issue(3) :751-760.DOI:10.13271/j.mpb.022.000751

基于转录组数据的绢毛委陵菜MYB转录因子家族分析

Analysis of the MYB Transcription Factor Family of Potentilla sericata Based on Transcriptome Data

刘振 1武俊刚 1李响 1夏威 1秦建 1刘庆鹏 1王学刚 1范卫芳 2吴建慧2
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作者信息

  • 1. 龙建路桥股份有限公司,哈尔滨,150000
  • 2. 东北林业大学园林学院,哈尔滨,150000
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摘要

利用生物信息学方法,从绢毛委陵菜转录组数据中筛选出63个MYB转录因子,分析了它们的转录因子家族序列特征、理化性质、保守结构域、亚细胞定位和进化关系.结果表明,筛选出的63个绢毛委陵菜转录因子,其中1R-MYB类22个、R2R3-MYB类37个、3R-MYB类3个、4R-MYB类1个.结构域分析表明其具有典型的W型R2R3保守基序;绢毛委陵菜的所有MYB基因都是亲水性不稳定蛋白;而亚细胞定位预测都位于细胞核内;系统进化分析将绢毛委陵菜R2R3-MYB类蛋白分为22个亚家族,其中参与调控重金属镉相关的拟南芥同源基因主要属于S20亚族.研究结果为深入探究绢毛委陵菜MYB基因在调控重金属镉过程中发挥的功能提供基础资料.

Abstract

Sixty-three MYB transcription factors were extracted from the Potentilla sericea transcriptome database by bioinformatics methods,and there was a comprehensive analysis of the sequence characteristics,physical and chemical properties,conserved domains,evolutionary relationships and cell locationat the same time which aim to screen out those genes that responses to Cd stress.The results showed that 63 MYB genes were identified in Potentilla sericea,37 of them belonged to R2R3-MYB,22 of them belonged to 1R-MYB,3 of them belonged to 3R-MYB and 1 of them belonged to 4R-MYB.Furthermore structural domain analysis showed that they have typical W type R2R3 conservative motifs;in the mean time,all those MYB proteins of Potentilla sericea were unstably hydrophilic proteins;subcellular localization prediction showed that MYB proteins of Potentilla sericea were all localized in nucleus.The phylogenetic of MYB proteins of Potentilla sericea were divided into 22 subfamilies according to the classification method of Arabidopsis family.The regulating genes related to heavy metal cadmium belong to the S20 subfamilies.This study showed that MYB genes of Potentilla sericea play an important regulatory role in responses to heavy metal cadmium and providing basic information for further exploration of the function of MYB genes of Potentilla sericea.

关键词

绢毛委陵菜/MYB转录因子/生物学分析/镉胁迫

Key words

Potentilla sericea/MYB transcription factor/Bioinformation analysis/Cadmium stress

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基金项目

七台河市山水林田湖草桃山区域生态环境治理项目(6101020201)

出版年

2024
分子植物育种
海南省生物工程协会

分子植物育种

CSTPCD北大核心
影响因子:0.765
ISSN:1672-416X
参考文献量20
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