The genetic diversity of hybrid rice combinations of Huazhan series approved in Guangxi were analy-zed to provide theoretical basis for breeding high-quality hybrid rice combinations of Huazhan series.In this study,48 SSR Primers published in agricultural industry standard(NY/T1433-2014)were used to analyze genetic diversity,construct DNA fingerprints and screen specific markers of 13 Huazhan hybrid rice varieties approved in Guangxi.The results show that,the mean value allele number of Guangxi,Guangdong and Hunan were 2.67,1.85 and 2.52;the mean values of gene diversity index were 1.809 7,1.629 5 and 1.888 5;the mean values of Shannon's index av-erages were 0.652 3,0.472 0 and 0.682 2;and the percentages of PIC＞0.5 were 16.7％,16.7％and 29.2％.The re-sults showed that the degree of genetic diversity of Huazhan hybrid rice combinations in Guangxi and Hunan was higher than Guangdong.Cluster analysis showed that the genetic distance of some combinations were close,but the combinations of Guangxi,Guangdong and Hunan could be distinguished basically.This study provides a tech-nical support for breeding of Huazhan high quality combination,identification and classification of Huazhan high-quality combination.
关键词
恢复系华占/遗传多样性/指纹图谱/特异性标记
Key words
Restorer line Huazhan/Genetic diversity/Fingerprint/Specific marker
维普
万方数据