首页|广西审定的华占系列杂交稻组合遗传多样性分析

广西审定的华占系列杂交稻组合遗传多样性分析

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分析广西审定的华占系列杂交稻组合的遗传多样性,为培育华占优质组合提供理论依据.本研究利用农业行业标准(NY/T1433-2014)中公布的48对SSR引物,对广西审定的13个华占系列杂交水稻品种进行遗传多样性分析、DNA指纹图谱的构建及特异性标记的筛选.结果显示,广西、广东和湖南的等位基因数平均值分别为2.67、1.85和2.52,基因多样性指数平均值分别为1.809 7、1.629 5和1.888 5,Shannon's指数平均值分别为0.652 3、0.472 0和0.682 2,PIC值>0.5占比分别为16.7%、16.7%和29.2%,说明广西和湖南的华占系列杂交稻组合遗传多样性较丰富,而广东的华占系列杂交稻组合遗传多样性水平较低.聚类分析表明,虽然部分组合遗传距离较为接近,但基本上可将广西、广东和湖南配制的组合区分开.本研究为选育华占优质组合、鉴定及分类提供了技术支撑.
Genetic Diversity Analysis of Hybrid Rice Combinations of Huazhan Series Approved in Guangxi
The genetic diversity of hybrid rice combinations of Huazhan series approved in Guangxi were analy-zed to provide theoretical basis for breeding high-quality hybrid rice combinations of Huazhan series.In this study,48 SSR Primers published in agricultural industry standard(NY/T1433-2014)were used to analyze genetic diversity,construct DNA fingerprints and screen specific markers of 13 Huazhan hybrid rice varieties approved in Guangxi.The results show that,the mean value allele number of Guangxi,Guangdong and Hunan were 2.67,1.85 and 2.52;the mean values of gene diversity index were 1.809 7,1.629 5 and 1.888 5;the mean values of Shannon's index av-erages were 0.652 3,0.472 0 and 0.682 2;and the percentages of PIC>0.5 were 16.7%,16.7%and 29.2%.The re-sults showed that the degree of genetic diversity of Huazhan hybrid rice combinations in Guangxi and Hunan was higher than Guangdong.Cluster analysis showed that the genetic distance of some combinations were close,but the combinations of Guangxi,Guangdong and Hunan could be distinguished basically.This study provides a tech-nical support for breeding of Huazhan high quality combination,identification and classification of Huazhan high-quality combination.

Restorer line HuazhanGenetic diversityFingerprintSpecific marker

唐梅、孙富、卿冬进、黄鹂、夏秀忠、廖张波、唐忠平、何聪、卢宏琮

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广西壮族自治区农业科学院水稻研究所,广西水稻遗传育种重点实验室,广西水稻优质化育种研究人才小高地,南宁,530007

广西壮族自治区农业科学院种子质量检测中心,南宁,530007

广西壮族自治区种子管理站,南宁,530022

恢复系华占 遗传多样性 指纹图谱 特异性标记

广西科技基地和人才专项广西农业科学院基本科研业务专项广西农业科学院科技发展基金项目广西农业科学院稳定资助科研团队项目广西特色作物试验站项目

桂科AD20159088桂农科2020YM71桂农科2022JM24桂农科2021YT034TS202121

2024

分子植物育种
海南省生物工程协会

分子植物育种

CSTPCD北大核心
影响因子:0.765
ISSN:1672-416X
年,卷(期):2024.22(3)
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