The Molecular Mechanism of HaNAC2 Transgenic Tobacco Response to Drought and High Temperature Stresses
In order to analyze the molecular mechanism of stress resistance of related genes specifically expressed in Haloxylon ammodendron under stress conditions,analysis of HaNAC2 protein activation and dimer formation by yeast hybridization,and the physiological indexes of HaNAC2 transgenic tobacco under high temperature and drought stress were measured and analyzed by RNA-seq technology.The results showed that HaNAC2 had no transcriptional activation activity in yeast and could not form homodimers.Under high temperature and drought stress,HaNAC2 transgenic tobacco has less damage and stronger resistance to high temperature and drought stress than non-transgenic tobacco.The roots of HaNA C2 transgenic tobacco were more developed than those of wild type under drought stress.The number of up-regulated genes in phenylpropanoid biosynthesis and carbon metabolism pathway was the largest after drought stress in transgenic tobacco roots,and the number of up-regulated genes in phenylpropanoid biosynthesis and starch sucrose metabolism pathway was the largest after high temperature stress in leaves.In summary,HaNAC2 protein may participate in the biosynthesis of lignin by interacting with other proteins,so that plants can withstand drought and high temperature stress.The results lay a theoretical foundation for clari-fying the molecular mechanism of stress resistance of HaNAC2 transcription factors in Haloxylon ammodendron.
万方数据
维普
