Establishment of Embryo Regeneration System of Malus robusta Rehd.
In order to establish an efficient embryo regeneration system of Malus robusta Rehd.,this study tested and analyzed the factors affecting the sterilization of Malus robusta Rehd seeds,embryo induction,tissue culture seedling proliferation,rooting,and leaf callus induction.The results show that the optimal disinfectant is 75％etha-nol/30 s+10％NaClO2/10 min before seed coat removal,and 10％NaC1O2/1 min after seed coat removal,which can reduce the contamination rate of seeds 0,and the highest germination rate is 93％;the optimal growth medium ratio for tissue culture seedlings is MS+2.0 mg/L 6-BA+0.1 mg/L NAA,the growth coefficient is 4.11,and the seedling height is 2.57 cm;the optimum rooting medium formula for tissue culture seedlings is 1/2MS+0.7 mg/L IBA+0.5 mg/L NAA,the rooting rate of tissue cultured seedlings reaches 100％after culture,the average root length is 7.97 cm,the number of main roots is 8.33,and the root surface area is 54.71 cm2,the number of root tips is 572.27,and the average plant height is 3.42 cm;the optimal medium ratio for leaf callus induction is MS+0.1 mg/L TDZ+1.0 mg/L NAA and MS+2.5 mg/L 2,4-D,the induction rate was 94.70％and 91.33％.This experiment provides a reference for the rapid propagation of Malus robusta Rehd..
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