GISH and FISH Karyotype Analysis of the Chromosomes in Aegilops ventricosa
The chromosome karyotype of Aegilops ventricosa was analyzed to understand its composition and characteristics by genomic in situ hybridization(GISH)and fluorescence in situ hybridization(FISH)in the present study.It would lay the cytological foundation for wheat genetics and breeding.The results showed the chromo-somes of the D and N genomes were clearly distinguished,and two pairs of translocation chromosomes between the D and N genomes were found by the GISH analysis.Oligo-pAsl-2 red signals were strong and abundant,and most of them were distributed at the telomeres.Oligo-pSc119.2-1 green signals on the N-genome chromosomes were more than those on the D genome.The(GAA)7 red signals were strong and abundant,and distributed mainly at the centromeres.The(GAA)7 signals on the N-genome chromosomes were significantly stronger than those on the D genome,which showed large area signals.Its FISH karyotype indicated that the translocated chromosomes were T5NL·3DL and T5NS·3DS,respectively.The FISH karyotype of the D-genome chromosomes was similar to that of the previous reports of the genome D in A.ventricosa and Triticum aestivum,but that of their N-genome chromosomes was quite different.The FISH karyotype and translocation chromosomes were different between this study and previous reports.Therefore,this material may be new Aegilops ventricosa germplasm.The results would provide the reference for the breeding of wheat new germplasm with A.ventricosa chromosome or fragment,and broadening wheat genetic background.
万方数据
维普
