Cloning and Function Analysis of TaMKK2 Gene from Wheat
Wheat experiences multiple abiotic stresses during its reproductive cycle,which restrict the growth and development of wheat.In order to improve stress resistance of new wheat cultivars,the MKK2 gene,tentatively named as TaMKK2,was bioinformatically isolated and the encoded protein was analyzed by homologous cloning based on MAPK stress resistance gene development in a pilot experiment using a new winter wheat cultivar'Xin-dong43'as well as subcellular localization and prokaryotic expression analysis.The results showed that the cDNA sequence was 531 bp in length,contained a complete open reading frame,encoded 176 amino acids,had a relative molecular weight of approximately 20.07 kD,and had an isoelectric point of 8.37,is close to the relative of the MKK2 gene of Cynanchum capri,Dioscorea brachypodium;theoretically localized to the nucleus,however,experi-mental manipulations failed to detect a fluorescent signal;the protein of interest in each induction condition was present as inclusion bodies,and was purified to obtain a protein of interest with a size of 20.07 kD after renatura-tion.The results of this study provide a theoretical basis for wheat to cope with abiotic stresses as well as to im-prove stress resistance in plants using gene breeding techniques.
NETL
NSTL
万方数据
维普
